Nyren’s sequencing method earned the name “pyrosequencing” since it revolved around the production of pyrophosphate. At first, pyrosequencing could sequence only short DNA snippets, with a few nucleotides. In 1996, however, Nyren’s lab demonstrated sequencing of up to 15 bases by using a modified “A” nucleotide to reduce their signal-to-noise ratio.7 In 1998, they increased this to 34 bases by adding another enzyme, called apyrase, to the mix; apyrase degraded unincorporated nucleotides, removing the need for constant wash steps.
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第九十七条 船舶在装货港开航前，托运人可以要求解除合同。但是，除合同另有约定外，托运人应当向承运人支付约定运费的一半；货物已经装船的，并应当负担装货、卸货和其他与此有关的费用。
While U.S. President Donald Trump expressed interest in eliminating daylight time shortly after his 2024 election, Congress has not passed the necessary legislation.